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Promega improm-ii™ 5× reaction buffer
Improm Ii™ 5× Reaction Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/improm-ii+5%C3%97+reaction+buffer/impromii+5%C3%97+reaction+buffer/us11944102-220-38-42
Average 90 stars, based on 1 article reviews
improm-ii™ 5× reaction buffer - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Reverse Transcription:

Article Title: Binding to SMN2 pre-mRNA-protein complex elicits specificity for small molecule splicing modifiers
Article Snippet: In a separate tube, 6.6 μl nuclease-free water were mixed with 4 μl ImProm-II 5× reaction buffer (Promega, M289C), 2.4 μl MgCl 2 (25 mM), 1 μl dNTP mix (10 mM), and 1 μl ImProm-II reverse transcriptase (Promega, M314C).

Article Title: Influence of adipocyte size and adipose depot on the number of adipose tissue macrophages and the expression of adipokines in dairy cows at the end of pregnancy.
Article Snippet: Afterward, 4 μL of Improm-II 5× reaction buffer, 2.4 μL of MgCl2 (25 mM), 1 μL of dNTP Mix (10 mM each; Bioline Reagents, London, UK), and 1 μL of Improm-II reverse transcriptase were added (Improm-II cDNA synthesis kit, Promega).

Article Title: Cholinergic Receptor and Cyclic Stretch-Mediated Inflammatory Gene Expression in Intact ASM
Article Snippet: The following reagents (Promega) were then added to the tube for reverse transcription, giving a final volume of 20 μl: 4 μl of ImProm-II 5× reaction buffer, 2.8 μl of 25 mM MgCl 2 (final concentration 3.5 mM), 1 μl of 10 mM dNTP mix (final concentration 0.5 mM), 20 units of recombinant RNasin, 1 μl of ImProm-II reverse transcriptase, 1 μl of 25 mM DTT (final concentration 1.25 mM), 2 μg of acetylated-BSA, and 2.7 μl of nuclease-free water.

Article Title: Matrix metalloproteinase-2 and -9 activities in the human lens epithelial cells and serum of steroid induced posterior subcapsular cataracts
Article Snippet: One microgram of total RNA was reverse transcribed at 25 °C for 5 min, 42 °C for 60 min, and 70 °C for 5 min in 20 μl of 0.5 μg of oligo (dT), 20 pmol primer, 3 mM of MgCl 2 , 1 mM of dNTPs, 4 μl of Improm-II TM 5× reaction buffer (Promega, Madison, WI), 1 unit of Recombinant RNasin ® RNase Inhibitor and 1 μl of Improm-II TM Reverse Transcriptase (Promega).

Article Title: Distinct neutrophil C5a receptor inflammatory events in cows initiated by chemoattractant C5a and lipopolysaccharide around parturition and in mid lactation.
Article Snippet: Then, 4 μL of Improm-II 5× reaction buffer (Promega), 2.4 μL of MgCl2 (25 mM), 1 μL of dNTP Mix (10 mM each; Bioline), and 1 μL of Improm-II reverse transcriptase (20 U/μL; Promega) were added.

Article Title: Functional Characterization of a Newly Identified Group B Streptococcus Pullulanase Eliciting Antibodies Able to Prevent Alpha-Glucans Degradation
Article Snippet: The mixture was used in a 20-μl (total volume) cDNA synthesis reaction mixture comprising 4 μl of Improm-II 5× reaction buffer (Promega), 2.4 μl MgCl 2 at 25 mM, 2 μl of dNTP mix (each dNTP at 2.5 mM), 0.25 μl of Rnasin RNase inhibitor (Promega) and 1 μl of Improm-II reverse transcriptase.

cDNA Synthesis:

Article Title: Binding to SMN2 pre-mRNA-protein complex elicits specificity for small molecule splicing modifiers
Article Snippet: In a separate tube, 6.6 μl nuclease-free water were mixed with 4 μl ImProm-II 5× reaction buffer (Promega, M289C), 2.4 μl MgCl 2 (25 mM), 1 μl dNTP mix (10 mM), and 1 μl ImProm-II reverse transcriptase (Promega, M314C).

Article Title: Influence of adipocyte size and adipose depot on the number of adipose tissue macrophages and the expression of adipokines in dairy cows at the end of pregnancy.
Article Snippet: Afterward, 4 μL of Improm-II 5× reaction buffer, 2.4 μL of MgCl2 (25 mM), 1 μL of dNTP Mix (10 mM each; Bioline Reagents, London, UK), and 1 μL of Improm-II reverse transcriptase were added (Improm-II cDNA synthesis kit, Promega).

Article Title: Cholinergic Receptor and Cyclic Stretch-Mediated Inflammatory Gene Expression in Intact ASM
Article Snippet: The following reagents (Promega) were then added to the tube for reverse transcription, giving a final volume of 20 μl: 4 μl of ImProm-II 5× reaction buffer, 2.8 μl of 25 mM MgCl 2 (final concentration 3.5 mM), 1 μl of 10 mM dNTP mix (final concentration 0.5 mM), 20 units of recombinant RNasin, 1 μl of ImProm-II reverse transcriptase, 1 μl of 25 mM DTT (final concentration 1.25 mM), 2 μg of acetylated-BSA, and 2.7 μl of nuclease-free water.

Article Title: Matrix metalloproteinase-2 and -9 activities in the human lens epithelial cells and serum of steroid induced posterior subcapsular cataracts
Article Snippet: One microgram of total RNA was reverse transcribed at 25 °C for 5 min, 42 °C for 60 min, and 70 °C for 5 min in 20 μl of 0.5 μg of oligo (dT), 20 pmol primer, 3 mM of MgCl 2 , 1 mM of dNTPs, 4 μl of Improm-II TM 5× reaction buffer (Promega, Madison, WI), 1 unit of Recombinant RNasin ® RNase Inhibitor and 1 μl of Improm-II TM Reverse Transcriptase (Promega).

Article Title: Distinct neutrophil C5a receptor inflammatory events in cows initiated by chemoattractant C5a and lipopolysaccharide around parturition and in mid lactation.
Article Snippet: Then, 4 μL of Improm-II 5× reaction buffer (Promega), 2.4 μL of MgCl2 (25 mM), 1 μL of dNTP Mix (10 mM each; Bioline), and 1 μL of Improm-II reverse transcriptase (20 U/μL; Promega) were added.

Article Title: Functional Characterization of a Newly Identified Group B Streptococcus Pullulanase Eliciting Antibodies Able to Prevent Alpha-Glucans Degradation
Article Snippet: The mixture was used in a 20-μl (total volume) cDNA synthesis reaction mixture comprising 4 μl of Improm-II 5× reaction buffer (Promega), 2.4 μl MgCl 2 at 25 mM, 2 μl of dNTP mix (each dNTP at 2.5 mM), 0.25 μl of Rnasin RNase inhibitor (Promega) and 1 μl of Improm-II reverse transcriptase.

Recombinant:

Article Title: Binding to SMN2 pre-mRNA-protein complex elicits specificity for small molecule splicing modifiers
Article Snippet: In a separate tube, 6.6 μl nuclease-free water were mixed with 4 μl ImProm-II 5× reaction buffer (Promega, M289C), 2.4 μl MgCl 2 (25 mM), 1 μl dNTP mix (10 mM), and 1 μl ImProm-II reverse transcriptase (Promega, M314C).

Article Title: Influence of adipocyte size and adipose depot on the number of adipose tissue macrophages and the expression of adipokines in dairy cows at the end of pregnancy.
Article Snippet: Afterward, 4 μL of Improm-II 5× reaction buffer, 2.4 μL of MgCl2 (25 mM), 1 μL of dNTP Mix (10 mM each; Bioline Reagents, London, UK), and 1 μL of Improm-II reverse transcriptase were added (Improm-II cDNA synthesis kit, Promega).

Article Title: Cholinergic Receptor and Cyclic Stretch-Mediated Inflammatory Gene Expression in Intact ASM
Article Snippet: The following reagents (Promega) were then added to the tube for reverse transcription, giving a final volume of 20 μl: 4 μl of ImProm-II 5× reaction buffer, 2.8 μl of 25 mM MgCl 2 (final concentration 3.5 mM), 1 μl of 10 mM dNTP mix (final concentration 0.5 mM), 20 units of recombinant RNasin, 1 μl of ImProm-II reverse transcriptase, 1 μl of 25 mM DTT (final concentration 1.25 mM), 2 μg of acetylated-BSA, and 2.7 μl of nuclease-free water.

Article Title: Matrix metalloproteinase-2 and -9 activities in the human lens epithelial cells and serum of steroid induced posterior subcapsular cataracts
Article Snippet: One microgram of total RNA was reverse transcribed at 25 °C for 5 min, 42 °C for 60 min, and 70 °C for 5 min in 20 μl of 0.5 μg of oligo (dT), 20 pmol primer, 3 mM of MgCl 2 , 1 mM of dNTPs, 4 μl of Improm-II TM 5× reaction buffer (Promega, Madison, WI), 1 unit of Recombinant RNasin ® RNase Inhibitor and 1 μl of Improm-II TM Reverse Transcriptase (Promega).

Article Title: Distinct neutrophil C5a receptor inflammatory events in cows initiated by chemoattractant C5a and lipopolysaccharide around parturition and in mid lactation.
Article Snippet: Then, 4 μL of Improm-II 5× reaction buffer (Promega), 2.4 μL of MgCl2 (25 mM), 1 μL of dNTP Mix (10 mM each; Bioline), and 1 μL of Improm-II reverse transcriptase (20 U/μL; Promega) were added.

Article Title: Functional Characterization of a Newly Identified Group B Streptococcus Pullulanase Eliciting Antibodies Able to Prevent Alpha-Glucans Degradation
Article Snippet: The mixture was used in a 20-μl (total volume) cDNA synthesis reaction mixture comprising 4 μl of Improm-II 5× reaction buffer (Promega), 2.4 μl MgCl 2 at 25 mM, 2 μl of dNTP mix (each dNTP at 2.5 mM), 0.25 μl of Rnasin RNase inhibitor (Promega) and 1 μl of Improm-II reverse transcriptase.

Concentration Assay:

Article Title: Binding to SMN2 pre-mRNA-protein complex elicits specificity for small molecule splicing modifiers
Article Snippet: In a separate tube, 6.6 μl nuclease-free water were mixed with 4 μl ImProm-II 5× reaction buffer (Promega, M289C), 2.4 μl MgCl 2 (25 mM), 1 μl dNTP mix (10 mM), and 1 μl ImProm-II reverse transcriptase (Promega, M314C).

Article Title: Influence of adipocyte size and adipose depot on the number of adipose tissue macrophages and the expression of adipokines in dairy cows at the end of pregnancy.
Article Snippet: Afterward, 4 μL of Improm-II 5× reaction buffer, 2.4 μL of MgCl2 (25 mM), 1 μL of dNTP Mix (10 mM each; Bioline Reagents, London, UK), and 1 μL of Improm-II reverse transcriptase were added (Improm-II cDNA synthesis kit, Promega).

Article Title: Cholinergic Receptor and Cyclic Stretch-Mediated Inflammatory Gene Expression in Intact ASM
Article Snippet: The following reagents (Promega) were then added to the tube for reverse transcription, giving a final volume of 20 μl: 4 μl of ImProm-II 5× reaction buffer, 2.8 μl of 25 mM MgCl 2 (final concentration 3.5 mM), 1 μl of 10 mM dNTP mix (final concentration 0.5 mM), 20 units of recombinant RNasin, 1 μl of ImProm-II reverse transcriptase, 1 μl of 25 mM DTT (final concentration 1.25 mM), 2 μg of acetylated-BSA, and 2.7 μl of nuclease-free water.

Article Title: Matrix metalloproteinase-2 and -9 activities in the human lens epithelial cells and serum of steroid induced posterior subcapsular cataracts
Article Snippet: One microgram of total RNA was reverse transcribed at 25 °C for 5 min, 42 °C for 60 min, and 70 °C for 5 min in 20 μl of 0.5 μg of oligo (dT), 20 pmol primer, 3 mM of MgCl 2 , 1 mM of dNTPs, 4 μl of Improm-II TM 5× reaction buffer (Promega, Madison, WI), 1 unit of Recombinant RNasin ® RNase Inhibitor and 1 μl of Improm-II TM Reverse Transcriptase (Promega).

Article Title: Distinct neutrophil C5a receptor inflammatory events in cows initiated by chemoattractant C5a and lipopolysaccharide around parturition and in mid lactation.
Article Snippet: Then, 4 μL of Improm-II 5× reaction buffer (Promega), 2.4 μL of MgCl2 (25 mM), 1 μL of dNTP Mix (10 mM each; Bioline), and 1 μL of Improm-II reverse transcriptase (20 U/μL; Promega) were added.

Article Title: Functional Characterization of a Newly Identified Group B Streptococcus Pullulanase Eliciting Antibodies Able to Prevent Alpha-Glucans Degradation
Article Snippet: The mixture was used in a 20-μl (total volume) cDNA synthesis reaction mixture comprising 4 μl of Improm-II 5× reaction buffer (Promega), 2.4 μl MgCl 2 at 25 mM, 2 μl of dNTP mix (each dNTP at 2.5 mM), 0.25 μl of Rnasin RNase inhibitor (Promega) and 1 μl of Improm-II reverse transcriptase.



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